40~50kms?
That's awesome capacity for a 16ah battery.
Again I am sure your battery has optimum capacity.
Be careful with the 1ah test because draining them slowly in such a way the ebike bms is not designed to protect the lithium in this fashion. The ebike
BMS usually is designed to LVC with higher loads and you may over draw the capacity too close to the cells capability.
It should be fine but really you should be satisfied with the first test with 5 amp load.
Remember LVC is supposed to cut you battery from draining 16ah from the battery if you were to get 16ah from a LVC protected 16ah battery then I would class this as a 20+ah battery. Remember 16ah running time on a 16ah lithium battery = dead battery, caput, finished, zero more cycles po0, Deathpo0 ..
It doesn't make sense to get 16ah from a 16ah LVC protected lithium battery.
LOL. A dead LiFePo4 = Po0.