Hi Ron,
Even without activating regen, the motor still generates voltage, and the voltage being generated will be directly proportional to the motor's rpm.
If the motor's rpm exceeds its maximum unloaded rpm, then the voltage being generated by the motor will be greater than the battery voltage, and this will cause current to flow from the motor into the battery.
The regenerative braking current is usually a lot higher than the residual generated current that naturally occurs when the motor's rpm exceeds the maximum unloaded motor rpm.
As an example, if the maximum unloaded speed of the motor at full throttle is 35mph, then the motor will automatically begin to generate current if the bike exceeds 35mph down a steep hill.
This is why I do not recommend using the bike at high speed without a battery, as there would be nowhere for the excess voltage to go, and if the voltage climbs too high, it could easily exceed the maximum safe voltage limit of some of the electrical components in the controller.
Alan