Tom,
It won't be the controller voltage setting as you is using a 48V battery, and the motor should run with the controller set to 24V, 36V, or 48V with that battery.
As Aliasssss has already mentioned, both of the brake levers need to be fully released for the motor to work, and because you don't have any brake cables fitted, both of your levers have the cable attachment pieces stuck between the lever and the lever mounting bracket, which is preventing the levers from releasing fully.
This will definitely cause the brake switch contacts to close and the motor will be unable to run until both brake switches are open
(lever fully released).
I would strongly recommend testing the motor with the rear end of the scooter placed on blocks to keep the wheel off the ground, just in case there is an intermittent problem with one of the throttle connections and the motor decides to suddenly start.
Wanting to see the wheel go round is understandable, but watching the scooter suddenly take off at full power on its own might not be quite so satisfying.
Alan